Advertisement
Research Article

Initial Genomics of the Human Nucleolus

  • Attila Németh,

    Affiliation: Department of Biochemistry III, University of Regensburg, Regensburg, Germany

    X
  • Ana Conesa,

    Affiliation: Department of Bioinformatics and Genomics, Centro de Investigación Príncipe Felipe, Valencia, Spain

    X
  • Javier Santoyo-Lopez,

    Affiliation: Department of Bioinformatics and Genomics, Centro de Investigación Príncipe Felipe, Valencia, Spain

    X
  • Ignacio Medina,

    Affiliation: Department of Bioinformatics and Genomics, Centro de Investigación Príncipe Felipe, Valencia, Spain

    X
  • David Montaner,

    Affiliation: Department of Bioinformatics and Genomics, Centro de Investigación Príncipe Felipe, Valencia, Spain

    X
  • Bálint Péterfia,

    Affiliation: Department of Biochemistry III, University of Regensburg, Regensburg, Germany

    X
  • Irina Solovei,

    Affiliation: Department of Biology II, Ludwig-Maximilians University of Munich, Planegg-Martinsried, Germany

    X
  • Thomas Cremer,

    Affiliation: Department of Biology II, Ludwig-Maximilians University of Munich, Planegg-Martinsried, Germany

    X
  • Joaquin Dopazo,

    Affiliation: Department of Bioinformatics and Genomics, Centro de Investigación Príncipe Felipe, Valencia, Spain

    X
  • Gernot Längst mail

    gernot.laengst@vkl.uni-regensburg.de

    Affiliation: Department of Biochemistry III, University of Regensburg, Regensburg, Germany

    X
  • Published: March 26, 2010
  • DOI: 10.1371/journal.pgen.1000889
  • Published in PLOS Genetics

Reader Comments (2)

Post a new comment on this article

Re: Histone marks of NAD

Posted by CheungCL on 14 Apr 2010 at 04:50 GMT

I supposed the author retrieved the histone modification data from the reference [34-36], however, the ChIP-seq of the histone modification was performed in CD4+ cell. I am just wondering how robust is the evidence of NAD region is enriched with repressive marks (H3K27me3...etc) and depleted of H3K4me1 in NAD... when the "cross-comparison" using histone mark from CD4+ in HeLa NAD regions may not be valid.

No competing interests declared.

RE: Re: Histone marks of NAD

glaengst replied to CheungCL on 15 Apr 2010 at 07:29 GMT

Dear colleague,
we expect to see cell-type specific differences rather at the level of individual NADs (i.e. the association is dynamic) than at the level of globally analyzed chromatin features. However, this should be experimentally tested. We were looking for HeLa histone modification datasets as well, and analyzed the
available ones (H3K27Me3). The result shows that there is an increase of this histone mark in the NADs of both cell lines. Please find the detailed description of the analysis in the legend of Table S6.

No competing interests declared.