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Research Article

A Microfluidic Device for Temporally Controlled Gene Expression and Long-Term Fluorescent Imaging in Unperturbed Dividing Yeast Cells

  • Gilles Charvin mail,

    To whom correspondence should be addressed. E-mail: gilles.charvin@gmail.com

    Affiliation: Center For Studies in Physics and Biology, The Rockefeller University, New York, New York, United States of America

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  • Frederick R. Cross,

    Affiliation: The Rockefeller University, New York, New York, United States of America

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  • Eric D. Siggia

    Affiliation: Center For Studies in Physics and Biology, The Rockefeller University, New York, New York, United States of America

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  • Published: January 23, 2008
  • DOI: 10.1371/journal.pone.0001468
  • Published in PLOS ONE

Reader Comments (4)

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added citation to Groisman et al. paper - See commentary for details

Posted by gcharvin on 05 Feb 2008 at 00:29 GMT

Yeast have been confined and imaged in microfluidic chambers
http://plosone.org/article/info:doi/10.1371/journal.pone.0001468#article1.body1.sec1.p2

A. Groisman, C. Lobo, H. Cho, K. J. Campbell, Y. S. Dufour, A. M. Stevens and A. Levchenko, "A Microfluidic Chemostat for Experiments with bacterial and yeast cells", Nature Methods 2, pp. 685-689 (2005)