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Research Article

Promotion of Reprogramming to Ground State Pluripotency by Signal Inhibition

  • Jose Silva equal contributor mail,

    equal contributor Contributed equally to this work with: Jose Silva, Ornella Barrandon

    To whom correspondence should be addressed. E-mail: jcs64@cscr.cam.ac.uk (JS); austin.smith@cscr.cam.ac.uk (AS)

    Affiliations: Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, United Kingdom, Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom

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  • Ornella Barrandon equal contributor,

    equal contributor Contributed equally to this work with: Jose Silva, Ornella Barrandon

    Affiliations: Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, United Kingdom, Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom

    X
  • Jennifer Nichols,

    Affiliations: Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, United Kingdom, Department of Physiology, Development, and Neuroscience, University of Cambridge, Cambridge, United Kingdom

    X
  • Jitsutaro Kawaguchi,

    Affiliations: Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, United Kingdom, Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom

    X
  • Thorold W Theunissen,

    Affiliations: Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, United Kingdom, Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom

    X
  • Austin Smith mail

    To whom correspondence should be addressed. E-mail: jcs64@cscr.cam.ac.uk (JS); austin.smith@cscr.cam.ac.uk (AS)

    Affiliations: Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, United Kingdom, Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom

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  • Published: October 21, 2008
  • DOI: 10.1371/journal.pbio.0060253
  • Published in PLOS Biology

About the Authors

Jose Silva, Ornella Barrandon, Jennifer Nichols, Jitsutaro Kawaguchi, Thorold W Theunissen, Austin Smith
Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, United Kingdom
Jose Silva, Ornella Barrandon, Jitsutaro Kawaguchi, Thorold W Theunissen, Austin Smith
Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom
Jennifer Nichols
Department of Physiology, Development, and Neuroscience, University of Cambridge, Cambridge, United Kingdom

Corresponding Authors

Competing Interests

A patent application based on this work has been filed with the United Kingdom patent office by the University of Cambridge (inventors Jose Silva and Austin Smith).

Author Contributions

JS and OB contributed equally to the generation and experimental characterisation of iPS cells. JN produced chimaeras. JK performed Southern hybridisation. and TT performed real time RT-PCR measurements. AS and JS designed and supervised the study and wrote the paper.