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Research Article

Poised Transcription Factories Prime Silent uPA Gene Prior to Activation

  • Carmelo Ferrai equal contributor,

    equal contributor Contributed equally to this work with: Carmelo Ferrai, Sheila Q. Xie

    Affiliations: Laboratory of Molecular Dynamics of the Nucleus, Division of Genetics and Cell Biology, S. Raffaele Scientific Institute, Milan, Italy, Medical Research Council Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital Campus, London, United Kingdom

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  • Sheila Q. Xie equal contributor,

    equal contributor Contributed equally to this work with: Carmelo Ferrai, Sheila Q. Xie

    Affiliation: Medical Research Council Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital Campus, London, United Kingdom

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  • Paolo Luraghi,

    Affiliation: Laboratory of Molecular Dynamics of the Nucleus, Division of Genetics and Cell Biology, S. Raffaele Scientific Institute, Milan, Italy

    Current address: Institute for Cancer Research and Treatment (IRCC), Torino, Italy

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  • Davide Munari,

    Affiliation: Laboratory of Molecular Dynamics of the Nucleus, Division of Genetics and Cell Biology, S. Raffaele Scientific Institute, Milan, Italy

    Current address: FIRC Institute of Molecular Oncology (IFOM), Milan, Italy

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  • Francisco Ramirez,

    Affiliation: South Ruislip, Middlesex, United Kingdom

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  • Miguel R. Branco,

    Affiliation: Medical Research Council Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital Campus, London, United Kingdom

    Current address: The Babraham Institute, Babraham Research Campus, Cambridge, United Kingdom

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  • Ana Pombo mail,

    ana.pombo@csc.mrc.ac.uk (AP); crippa.massimo@hsr.it (MPC)

    Affiliation: Medical Research Council Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital Campus, London, United Kingdom

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  • Massimo P. Crippa mail

    ana.pombo@csc.mrc.ac.uk (AP); crippa.massimo@hsr.it (MPC)

    Affiliation: Laboratory of Molecular Dynamics of the Nucleus, Division of Genetics and Cell Biology, S. Raffaele Scientific Institute, Milan, Italy

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  • Published: January 05, 2010
  • DOI: 10.1371/journal.pbio.1000270
  • Published in PLOS Biology

About the Authors

Carmelo Ferrai, Paolo Luraghi, Davide Munari, Massimo P. Crippa
Laboratory of Molecular Dynamics of the Nucleus, Division of Genetics and Cell Biology, S. Raffaele Scientific Institute, Milan, Italy
Carmelo Ferrai, Sheila Q. Xie, Miguel R. Branco, Ana Pombo
Medical Research Council Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital Campus, London, United Kingdom
Francisco Ramirez
South Ruislip, Middlesex, United Kingdom

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

The author(s) have made the following declarations about their contributions: Conceived and designed the experiments: CF AP MPC. Performed the experiments: CF SQX PL DM. Analyzed the data: CF SQX FR AP MPC. Contributed reagents/materials/analysis tools: CF SQX MRB AP. Wrote the paper: CF SQX AP MPC. Performed the experiments on uPA expression (mRNA and protein), MN digestion of chromatin, MN-ChIP: CF PL. Performed immuno-cryoFISH experiments and analyses: SQX. Performed statistical analysis: FR AP.