Advertisement
Research Article

Maize Centromere Structure and Evolution: Sequence Analysis of Centromeres 2 and 5 Reveals Dynamic Loci Shaped Primarily by Retrotransposons

  • Thomas K. Wolfgruber equal contributor,

    equal contributor Contributed equally to this work with: Thomas K. Wolfgruber, Anupma Sharma, Kevin L. Schneider

    Affiliation: Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, Hawaii, United States of America

    X
  • Anupma Sharma equal contributor,

    equal contributor Contributed equally to this work with: Thomas K. Wolfgruber, Anupma Sharma, Kevin L. Schneider

    Affiliation: Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, Hawaii, United States of America

    X
  • Kevin L. Schneider equal contributor,

    equal contributor Contributed equally to this work with: Thomas K. Wolfgruber, Anupma Sharma, Kevin L. Schneider

    Affiliation: Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, Hawaii, United States of America

    X
  • Patrice S. Albert,

    Affiliation: Division of Biological Sciences, University of Missouri, Columbia, Missouri, United States of America

    X
  • Dal-Hoe Koo,

    Affiliation: Department of Horticulture, University of Wisconsin–Madison, Madison, Wisconsin, United States of America

    X
  • Jinghua Shi,

    Affiliation: Department of Genetics, University of Georgia, Athens, Georgia, United States of America

    X
  • Zhi Gao,

    Affiliation: Division of Biological Sciences, University of Missouri, Columbia, Missouri, United States of America

    X
  • Fangpu Han,

    Affiliation: Division of Biological Sciences, University of Missouri, Columbia, Missouri, United States of America

    X
  • Hyeran Lee,

    Affiliation: Department of Horticulture, University of Wisconsin–Madison, Madison, Wisconsin, United States of America

    X
  • Ronghui Xu,

    Affiliation: Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, Hawaii, United States of America

    X
  • Jamie Allison,

    Affiliation: Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, Hawaii, United States of America

    X
  • James A. Birchler,

    Affiliation: Division of Biological Sciences, University of Missouri, Columbia, Missouri, United States of America

    X
  • Jiming Jiang,

    Affiliation: Department of Horticulture, University of Wisconsin–Madison, Madison, Wisconsin, United States of America

    X
  • R. Kelly Dawe,

    Affiliation: Department of Genetics, University of Georgia, Athens, Georgia, United States of America

    X
  • Gernot G. Presting mail

    gernot@hawaii.edu

    Affiliation: Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, Hawaii, United States of America

    X
  • Published: November 20, 2009
  • DOI: 10.1371/journal.pgen.1000743
  • Published in PLOS Genetics

About the Authors

Thomas K. Wolfgruber, Anupma Sharma, Kevin L. Schneider, Ronghui Xu, Jamie Allison, Gernot G. Presting
Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, Hawaii, United States of America
Patrice S. Albert, Zhi Gao, Fangpu Han, James A. Birchler
Division of Biological Sciences, University of Missouri, Columbia, Missouri, United States of America
Dal-Hoe Koo, Hyeran Lee, Jiming Jiang
Department of Horticulture, University of Wisconsin–Madison, Madison, Wisconsin, United States of America
Jinghua Shi, R. Kelly Dawe
Department of Genetics, University of Georgia, Athens, Georgia, United States of America

Corresponding Author

Email: gernot@hawaii.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: TKW AS KLS JS JAB JJ RKD GGP. Performed the experiments: TKW AS KLS PSA DHK JS HL ZG FH RX JA GGP. Analyzed the data: TKW AS KLS PSA DHK JS JAB JJ RKD GGP. Contributed reagents/materials/analysis tools: TKW. Wrote the paper: TKW AS KLS GGP. Derived and mapped RJ and OMA markers: TKW, AS. Performed ChIP enrichment studies: TKW. Identified retrotransposons and dated their insertion times: AS, KLS. Designed and cloned CRM-specific FISH probes: AS, RX, JA. Mapped ChIP reads to maize BACs and reference chromosomes: KLS. Generated the BAC physical map: KLS. Performed FISH analysis: PSA, ZG, FH, JAB. Derived and mapped TD markers: JS, RKD. Performed chromatin immunoprecipitation: HL, JJ.