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Research Article

High-Level Production of Amorpha-4,11-Diene, a Precursor of the Antimalarial Agent Artemisinin, in Escherichia coli

  • Hiroko Tsuruta,

    Affiliation: Amyris Biotechnologies, Emeryville, California, United States of America

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  • Christopher J. Paddon mail,

    Paddon@amyris.com

    Affiliation: Amyris Biotechnologies, Emeryville, California, United States of America

    X
  • Diana Eng,

    Affiliation: Amyris Biotechnologies, Emeryville, California, United States of America

    X
  • Jacob R. Lenihan,

    Affiliation: Amyris Biotechnologies, Emeryville, California, United States of America

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  • Tizita Horning,

    Affiliation: Amyris Biotechnologies, Emeryville, California, United States of America

    X
  • Larry C. Anthony,

    Affiliation: Amyris Biotechnologies, Emeryville, California, United States of America

    Current address: DuPont Central Research & Development, Experimental Station E328/345B, Wilmington, Delaware, United States of America

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  • Rika Regentin,

    Affiliation: Amyris Biotechnologies, Emeryville, California, United States of America

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  • Jay D. Keasling,

    Affiliations: Departments of Chemical Engineering and of Bioengineering, University of California, Berkeley, California, United States of America, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America, Joint BioEnergy Institute, Emeryville, California, United States of America

    X
  • Neil S. Renninger,

    Affiliation: Amyris Biotechnologies, Emeryville, California, United States of America

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  • Jack D. Newman

    Affiliation: Amyris Biotechnologies, Emeryville, California, United States of America

    X
  • Published: February 16, 2009
  • DOI: 10.1371/journal.pone.0004489
  • Published in PLOS ONE

About the Authors

Hiroko Tsuruta, Christopher J. Paddon, Diana Eng, Jacob R. Lenihan, Tizita Horning, Larry C. Anthony, Rika Regentin, Neil S. Renninger, Jack D. Newman
Amyris Biotechnologies, Emeryville, California, United States of America
Jay D. Keasling
Departments of Chemical Engineering and of Bioengineering, University of California, Berkeley, California, United States of America
Jay D. Keasling
Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America
Jay D. Keasling
Joint BioEnergy Institute, Emeryville, California, United States of America

Corresponding Author

Email: Paddon@amyris.com

Competing Interests

All authors are shareholders or hold share options in Amyris Biotechnologies.

Author Contributions

Conceived and designed the experiments: HT CJP JRL TH LCA RR NSR JDN. Performed the experiments: HT CJP DE JRL TH LCA. Analyzed the data: HT CJP DE JRL TH LCA RR NSR JDN. Contributed reagents/materials/analysis tools: JDK. Wrote the paper: HT CJP.