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Research Article

A Modular Cloning System for Standardized Assembly of Multigene Constructs

  • Ernst Weber equal contributor,

    equal contributor Contributed equally to this work with: Ernst Weber, Carola Engler

    Affiliation: Icon Genetics GmbH, Halle/Saale, Germany

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  • Carola Engler equal contributor,

    equal contributor Contributed equally to this work with: Ernst Weber, Carola Engler

    Affiliation: Icon Genetics GmbH, Halle/Saale, Germany

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  • Ramona Gruetzner,

    Affiliation: Icon Genetics GmbH, Halle/Saale, Germany

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  • Stefan Werner,

    Affiliation: Icon Genetics GmbH, Halle/Saale, Germany

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  • Sylvestre Marillonnet mail

    marillonnet@icongenetics.de

    Affiliation: Icon Genetics GmbH, Halle/Saale, Germany

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  • Published: February 18, 2011
  • DOI: 10.1371/journal.pone.0016765
  • Published in PLOS ONE

About the Authors

Ernst Weber, Carola Engler, Ramona Gruetzner, Stefan Werner, Sylvestre Marillonnet
Icon Genetics GmbH, Halle/Saale, Germany

Corresponding Author

Email: marillonnet@icongenetics.de

Competing Interests

EW, CE, RG, SW and SM are employees of Icon Genetics GmbH. Icon Genetics GmbH has filed a patent application on the vector system presented in this article. This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: SM. Performed the experiments: EW CE RG SW. Wrote the paper: EW SM.