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Research Article

A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes

  • Linda A. Amaral-Zettler mail,

    amaral@mbl.edu

    Affiliation: The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts, United States of America

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  • Elizabeth A. McCliment,

    Affiliation: The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts, United States of America

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  • Hugh W. Ducklow,

    Affiliation: The Ecosystems Center, Marine Biological Laboratory, Woods Hole, Massachusetts, United States of America

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  • Susan M. Huse

    Affiliation: The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts, United States of America

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  • Published: July 27, 2009
  • DOI: 10.1371/journal.pone.0006372
  • Published in PLOS ONE

About the Authors

Linda A. Amaral-Zettler, Elizabeth A. McCliment, Susan M. Huse
The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts, United States of America
Hugh W. Ducklow
The Ecosystems Center, Marine Biological Laboratory, Woods Hole, Massachusetts, United States of America

Corresponding Author

Email: amaral@mbl.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: LAZ EAM. Performed the experiments: EAM. Analyzed the data: LAZ EAM SMH. Contributed reagents/materials/analysis tools: LAZ. Wrote the paper: LAZ EAM HWD SMH.