Citation: Croll D, McDonald BA (2012) The Accessory Genome as a Cradle for Adaptive Evolution in Pathogens. PLoS Pathog 8(4): e1002608. doi:10.1371/journal.ppat.1002608
Editor: Joseph Heitman, Duke University Medical Center, United States of America
Published: April 26, 2012
Copyright: © 2012 Croll, McDonald. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by the Swiss Federal Institute of Technology (ETH), Zurich, and the Swiss National Science Foundation (SNF Grant 31003A_134755). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Pathogens offer many of the most fascinating and well-studied examples of evolution because their speed of adaptation allows observation of evolutionary change within human lifetimes. The rapid evolution observed in pathogenic viruses, bacteria, and fungi affect human, animal, and plant health globally. For example, influenza viruses regularly recombine genes affecting host range, infection pathways, and virulence prior to the emergence of deadly outbreaks . The emergence of antibiotic resistance in bacteria, including highly resistant Staphylococcus aureus strains and the re-emergence of resistant tuberculosis, is well documented , . The recent outbreak in Africa of wheat stem rust caused by the Ug99 pathotype of the fungus Puccinia graminis tritici surprised many plant pathologists, who thought this ancient pathogen was defeated during the green revolution . It is clear that pathogens engage host species in a constant race to evolve new defense mechanisms. By extension, we are constantly challenged to find new treatments and better containment measures to protect human health and essential crops.
Is There a Genetic Mechanism Enabling More Rapid Evolution in Pathogens?
Extensive work on the molecular basis of pathogen adaptation reveals several common themes. Pathogens usually have higher rates of reproduction and shorter generation times than their hosts. Large effective population sizes maintain genetic diversity while recombination or reassortment puts together favorable combinations of mutations. Switches in reproductive modes are frequent, as epidemics are often caused by an emerging clone that has fixed a favorable combination of alleles and gained the potential for rapid dissemination. However, the astonishing capacity of pathogens to evolve rapidly is not based solely on these life-history traits.
It appears that rapid adaptation to hosts and new environments is favored when pathogens carry a highly variable, rapidly mutating genomic compartment. For example, viruses can carry satellite RNAs  while toxins and antibiotic resistance factors are often encoded on plasmids in bacteria . Pathogenic fungi often possess highly variable regions that may be “lineage-specific” (i.e., restricted to a set of isolates) and cover entire chromosomes. Such chromosomes have been called accessory, supernumerary, B, or dispensable chromosomes. Their discovery dates back to 1907, with the very first observation of unusual chromosomes (in hemipteran insects) . We think that the term “accessory” provides the most neutral description of these chromosomes and the term is well aligned with the literature on bacterial pathogens. The rapidly evolving compartments of pathogen genomes have been associated with high mutation rates, the acquisition of foreign genes, copy number polymorphisms, and frequent ectopic recombination –. By comparison, the core genome carries essential genes that encode basal functions such as cellular metabolism and reproduction. Because of its essential functions, the core genome is thought to be shielded from high recombination and mutation rates and insertion of novel genes. Recent genomic comparisons have revealed that many pathogens share a strikingly compartmentalized genome, which we will refer to as the “2-speed genome” following Raffaele et al. .
Fungal Pathogens Carry an Extensive Set of Accessory Genomic Elements
Fungi possess sophisticated accessory genomic elements linked to pathogenicity that can span entire chromosomes. We will focus this review on these accessory chromosomes.
Nectria haematococca was the first discovered and probably best-studied fungus carrying accessory chromosomes. Accessory chromosome 14 carries a cluster of genes encoding detoxification of a phytoalexin produced by pea plants, enabling isolates carrying this chromosome to be virulent on peas , . Chromosome 14, as well as two other accessory chromosomes (15 and 17), show striking differences to the core chromosomes. A substantial fraction of the genes located on these chromosomes are more similar to the distantly related Aspergillus clade than to the more closely related Fusarium species . These accessory chromosomes are also enriched in transposable elements and gene duplications. The origin of N. haematococca accessory chromosomes remains unresolved, as it is unknown whether the chromosomes originated from ancient core chromosomes or whether interspecific transfers might have been involved .
A similar picture has emerged of the accessory chromosomes of the tomato pathogen Fusarium oxysporum (f. sp. lycopersici). Nearly 40% of the F. oxysporum genome was specific to the tomato-infecting lineage and lacked synteny with closely related species . In this study, accessory chromosomes contained the majority of transposable elements and genes were enriched in secreted proteins and virulence factors. Similar to what was found for N. haematococca, phylogenetic analyses of the genes on F. oxysporum accessory chromosomes point to horizontal transfer as the most likely origin. The consensus for a broad set of genes located on the accessory chromosomes points to an emergence coinciding with the root of the F. oxysporum, F. verticillioides, and F. graminearum clade but prior to the split with N. haematococca. Interestingly, F. oxysporum chromosome 14 can be exchanged among different strains despite the lack of sexual recombination .
Mycosphaerella graminicola, a major wheat pathogen that originated in the Fertile Crescent during the domestication of wheat, carries an especially rich set of accessory chromosomes ,  (Figure 1). M. graminicola maintains extensive chromosomal length polymorphism within populations, likely through the high degree of chromosomal rearrangements occurring during meiosis , . Similar to other fungi, the genes located on accessory chromosomes show only weak homology to genes found on core chromosomes or closely related species. The Mycosphaerella clade may provide an interesting model for the evolution of accessory chromosomes as closely related sister species carry sets of chromosomes of similar length. However, it is currently unknown whether these chromosomes are accessory or part of the core chromosome set. Interestingly, genes on accessory chromosomes with homologs in sister species showed an accelerated rate of evolution compared to the core genome .
Figure 1. Diversity in essential and accessory chromosomes in the Mycosphaerella clade.
Chromosomes of the outgroup species Septoria passerinii (S.p.), two isolates of Mycosphaerella S2, one isolate of Mycosphaerella S1, and three isolates of M. graminicola were visualized by pulsed-field gel electrophoresis (PFGE). Mycosphaerella S2 and S1 are found on wild grasses in Iran, the center of origin of M. graminicola, a major leaf pathogen of wheat . The size markers are Hansenula wingei chromosomes. The phylogenetic tree is based on a six-gene alignment.doi:10.1371/journal.ppat.1002608.g001
The Two-Speed Genome as a Fundamental Characteristic of Rapidly Evolving Pathogens
This brief survey of fungal pathogens suggests that at least some portions of the accessory chromosomes constitute the rapidly evolving segment of a genome that evolves at different speeds. Compartmentalization of the genome may provide several evolutionary advantages to the pathogen. Among the benefits of an accessory genome are the potential to acquire novel virulence factors that can expand host range. A novel gene function may evolve through gene or segmental duplication within the genome or through horizontal transfer. Depending on the mechanism of gene insertion or duplication, the novel gene may disrupt an existing, functional gene, influence gene transcription, and/or favor non-homologous recombination. The higher rate of substitutions and non-homologous recombination may be permissive only for chromosomes largely devoid of essential genes. Accessory chromosomes, therefore, provide an ideal “testing ground” for the evolution of new genes. A pathogen faced by a high heterogeneity among potential hosts may benefit from having virulence genes located on accessory chromosomes. A gene that is beneficial to the pathogen only on a particular host genotype but detrimental on other hosts may more rapidly increase or decrease in frequency in a population if it is located on an accessory chromosome.
A high mutation rate coupled with the expected lack of homologous pairings during meiosis for an accessory chromosome may lead to gradual decay of its chromosomal structure. Similar to the processes observed for the mating type loci in Cryptococcus neoformans and Neurospora tetrasperma , , as well as heteromorphic animal sex chromosomes , an accessory chromosome is expected to gradually decay in a population unless homologous pairings help purge deleterious mutations. This process, termed Muller's ratchet, was proposed to operate on animal accessory chromosomes . In fungal pathogens, large population sizes are expected to slow a ratchet process of degeneration. However, as many genes on accessory chromosomes are not likely to be under strong selection pressure, segmental deletions could be fixed either through founder events or selective sweeps affecting the pathogen populations. Hints of the decay of accessory chromosomes in fungi are found in M. graminicola, where several accessory chromosomes show large length variation segregating within populations . A largely unexplored aspect of accessory chromosomes is their capacity for meiotic drive (i.e., a transmission rate higher than expected under Mendelian segregation). The extensive literature on animal accessory chromosomes shows that meiotic drive is ubiquitous . The propensity of accessory chromosomes to be horizontally transferred under lab conditions in F. oxysporum and Alternaria alternata ,  may provide an analogous scenario to “selfish” drive transmission. Understanding the evolutionary dynamics of accessory chromosomes in fungi will require consideration of both the beneficial effects of virulence genes located on these chromosomes and their transmission characteristics through both meiosis and horizontal chromosome transfer.
Toward a More Comprehensive Understanding of Accessory Genomes in Pathogens
Recognizing common themes in the vast diversity of organisms carrying accessory genome elements enables us to refine our understanding of the genetic basis of rapid evolution in pathogens. But a central question remains largely unanswered: How are accessory genomic elements generated and how are they maintained through evolution?
In bacteria, important virulence traits and antibiotic resistance are often encoded on accessory genome elements such as plasmids. The potential for the evolution of antibiotic resistance and increased virulence is often directly related to the mobility of the genes encoding these traits among strains. Will similar trends emerge for the fungal accessory genomes? Do fungal clades with a propensity to carry accessory chromosomes (such as Fusarium or Mycosphaerella) pose a more significant threat for rapid evolution of virulence or drug resistance?
We would like to thank Beat Ruffner, Christine Grossen, and two anonymous reviewers for providing helpful comments on the manuscript, and Marcello Zala for providing the PFGE image.
- 1. Nelson MI, Holmes EC (2007) The evolution of epidemic influenza. Nat Rev Genet 8: 196–205. doi:10.1038/nrg2053.
- 2. Shah NS, Wright A, Bai G-H, Barrera L, Boulahbal F, et al. (2007) Worldwide emergence of extensively drug-resistant tuberculosis. Emerg Infect Dis 13: 380–387.
- 3. Harris SR, Feil EJ, Holden MTG, Quail MA, Nickerson EK, et al. (2010) Evolution of MRSA during hospital transmission and intercontinental spread. Science 327: 469–474. doi:10.1126/science.1182395.
- 4. Singh RP, Hodson DP, Huerta-Espino J, Jin Y, Bhavani S, et al. (2011) The emergence of Ug99 races of the stem rust fungus is a threat to world wheat production. Annu Rev Phytopathol 49: 465–481. doi:10.1146/annurev-phyto-072910-095423.
- 5. Hu C-C, Hsu Y-H, Lin N-S (2009) Satellite RNAs and Satellite Viruses of Plants. Viruses 1: 1325–1350. doi:10.3390/v1031325.
- 6. Robicsek A, Jacoby GA, Hooper DC (2006) The worldwide emergence of plasmid-mediated quinolone resistance. Lancet Infect Dis 6: 629–640.
- 7. Wilson EB (1907) The supernumerary chromosomes of Hemiptera. Science 26: 870–871.
- 8. Wittenberg AHJ, van der Lee TAJ, Ben M'barek S, Ware SB, Goodwin SB, et al. (2009) Meiosis drives extraordinary genome plasticity in the haploid fungal plant pathogen Mycosphaerella graminicola. PLoS ONE 4: e5863. doi:10.1371/journal.pone.0005863.
- 9. van de Wouw AP, Cozijnsen AJ, Hane JK, Brunner PC, McDonald BA, et al. (2010) Evolution of linked avirulence effectors in Leptosphaeria maculans is affected by genomic environment and exposure to resistance genes in host plants. PLoS Pathog 6: e1001180. doi:10.1371/journal.ppat.1001180.
- 10. Raffaele S, Farrer RA, Cano LM, Studholme DJ, MacLean D, et al. (2010) Genome evolution following host jumps in the Irish potato famine pathogen lineage. Science 330: 1540–1543. doi:10.1126/science.1193070.
- 11. Coleman JJ, Rounsley SD, Rodriguez-Carres M, Kuo A, Wasmann CC, et al. (2009) The genome of Nectria haematococca: contribution of supernumerary chromosomes to gene expansion. PLoS Genet 5: e1000618. doi:10.1371/journal.pgen.1000618.
- 12. Miao VPW, Covert SF, VanEtten HD (1991) A fungal gene for antibiotic resistance on a dispensable (“B”) chromosome. Science 254: 1773–1776.
- 13. Ma L-J, van der Does HC, Borkovich KA, Coleman JJ, Daboussi M-J, et al. (2010) Comparative genomics reveals mobile pathogenicity chromosomes in Fusarium. Nature 464: 367–373. doi:10.1038/nature08850.
- 14. Stukenbrock EH, Banke S, Javan-Nikkhah M, McDonald BA (2007) Origin and domestication of the fungal wheat pathogen Mycosphaerella graminicola via sympatric speciation. Mol Biol Evol 24: 398–411.
- 15. Goodwin SB, M'barek SB, Dhillon B, Wittenberg AHJ, Crane CF, et al. (2011) Finished genome of the fungal wheat pathogen Mycosphaerella graminicola reveals dispensome structure, chromosome plasticity, and stealth pathogenesis. PLoS Genet 7: e1002070. doi:10.1371/journal.pgen.1002070.
- 16. McDonald BA, Martinez J (1991) Chromosome Length Polymorphisms in a Septoria tritici Population. Curr Genet 19: 265–271.
- 17. Stukenbrock EH, Jørgensen FG, Zala M, Hansen TT, McDonald BA, et al. (2010) Whole-genome and chromosome evolution associated with host adaptation and speciation of the wheat pathogen Mycosphaerella graminicola. PLoS Genet 6: e1001189. doi:10.1371/journal.pgen.1001189.
- 18. Lengeler KB, Fox DS, Fraser JA, Allen A, Forrester K, et al. (2002) Mating-type locus of Cryptococcus neoformans: a step in the evolution of sex chromosomes. Eukaryot Cell 1: 704–718.
- 19. Ellison CE, Stajich JE, Jacobson DJ, Natvig DO, Lapidus A, et al. (2011) Massive changes in genome architecture accompany the transition to self-fertility in the filamentous fungus Neurospora tetrasperma. Genetics 189: 55–69. doi:10.1534/genetics.111.130690.
- 20. Charlesworth D, Charlesworth B, Marais G (2005) Steps in the evolution of heteromorphic sex chromosomes. Heredity 95: 118–128. doi:10.1038/sj.hdy.6800697.
- 21. Green DM (1990) Muller's Ratchet and the evolution of supernumerary chromosomes. Genome 33: 818–824.
- 22. Jones R (1991) B-Chromosome Drive. Am Nat 137: 430–442.
- 23. Akagi Y, Akamatsu H, Otani H, Kodama M (2009) Horizontal chromosome transfer, a mechanism for the evolution and differentiation of a plant-pathogenic fungus. Eukaryot Cell 8: 1732–1738. doi:10.1128/EC.00135-09.