Brachyspira hyodysenteriae is an anaerobic intestinal spirochete that colonizes the large intestine of pigs and causes swine dysentery, a disease of significant economic importance. The genome sequence of B. hyodysenteriae strain WA1 was determined, making it the first representative of the genus Brachyspira to be sequenced, and the seventeenth spirochete genome to be reported. The genome consisted of a circular 3,000,694 base pair (bp) chromosome, and a 35,940 bp circular plasmid that has not previously been described. The spirochete had 2,122 protein-coding sequences. Of the predicted proteins, more had similarities to proteins of the enteric Escherichia coli and Clostridium species than they did to proteins of other spirochetes. Many of these genes were associated with transport and metabolism, and they may have been gradually acquired through horizontal gene transfer in the environment of the large intestine. A reconstruction of central metabolic pathways identified a complete set of coding sequences for glycolysis, gluconeogenesis, a non-oxidative pentose phosphate pathway, nucleotide metabolism, lipooligosaccharide biosynthesis, and a respiratory electron transport chain. A notable finding was the presence on the plasmid of the genes involved in rhamnose biosynthesis. Potential virulence genes included those for 15 proteases and six hemolysins. Other adaptations to an enteric lifestyle included the presence of large numbers of genes associated with chemotaxis and motility. B. hyodysenteriae has diverged from other spirochetes in the process of accommodating to its habitat in the porcine large intestine.
Citation: Bellgard MI, Wanchanthuek P, La T, Ryan K, Moolhuijzen P, et al. (2009) Genome Sequence of the Pathogenic Intestinal Spirochete Brachyspira hyodysenteriae Reveals Adaptations to Its Lifestyle in the Porcine Large Intestine. PLoS ONE 4(3): e4641. doi:10.1371/journal.pone.0004641
Editor: Niyaz Ahmed, University of Hyderabad, India
Received: December 22, 2008; Accepted: January 6, 2009; Published: March 5, 2009
Copyright: © 2009 Bellgard et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This study was supported by the Australian Research Council and Novartis Animal Vaccines through Linkage grant number LP0348441 for Genomic sequencing and comparative genomic analysis for animal bacterial vaccine discovery, and by the Western Australian State Government in establishment of the WA Centre of Excellence for Comparative Genomics and support for this project. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Analysis of 16S rRNA gene sequences has shown that spirochetes form a distinct monophyletic phylum of bacteria, containing three families . Amongst these spirochetes there are four genera that contain important pathogenic species, these being Treponema, Borrelia, Leptospira and Brachyspira. The genus Brachyspira is made up of anaerobic spirochetes that colonize the large intestines of various species of animals and birds. Of the seven named Brachyspira species, some are commensals, whilst others are important pathogens. The species that has been studied in most detail is Brachyspira hyodysenteriae, the etiological agent of swine dysentery – a severe mucohemorrhagic colitis of pigs . This spirochete can survive transiently in the external environment, particularly in moist feces, and it may on occasion colonize other animal and bird species, but its main habitat is the porcine cecum and colon.
Despite the economic importance of swine dysentery and the need to control the disease, knowledge is lacking about metabolic and other adaptations that have allowed the spirochete to successfully colonize the complex and potentially hostile environment of the large intestine, and to induce disease . This situation is a reflection of the paucity of available genomic information about the spirochete, lack of genetic tools, and the difficulties involved in its genetic manipulation . B. hyodysenteriae is found associated with mucus in the lumen and crypts of the porcine cecum and colon, where it induces damage to underlying enterocytes and results in an outpouring of mucus and sloughing of areas of the epithelium. Both chemotaxis and motility are believed to be important in allowing B. hyodysenteriae to penetrate the mucus and associate with the gut mucosa . The spirochete shows chemotactic attraction to mucin , and penetrates mucus with a corkscrew-like motility. Disruptions introduced to B. hyodysenteriae flagella genes (flaA and flaB) reduce the spirochete's capacity to colonize and cause disease . B. hyodysenteriae is an anaerobe and its ability to colonize the colonic mucosa is enhanced by its NADH oxidase activity that protects it from oxygen toxicity . Local damage to colonic enterocytes may be caused by one or more hemolysins , whilst the endotoxic effects of lipooligosaccharide (LOS) in the cell envelope may contribute to the pathology observed in colonized swine .
In the current study the genome of B. hyodysenteriae strain WA1 (ATCC 49526) was sequenced and subjected to comparative genomic analysis with the aim of enhancing knowledge about adaptations in this spirochete that have allowed it to colonize the large intestine and induce disease.
Results and Discussion
B. hyodysenteriae general genome statistics
The genome of B. hyodysenteriae strain WA1 consisted of a single circular 3,000,694 base pair (bp) chromosome, and a 35,940 bp circular plasmid (Figure 1). This is the first confirmed report of the existence of a plasmid in B. hyodysenteriae. Compared to the genomes of other spirochetes, this arrangement was simpler than in Borrelia species where there is a linear chromosome and numerous circular or linear plasmids, and in Leptospira species where there are two or three major replicons. On the other hand, the sequenced Treponema species have a single circular chromosome and no plasmids. The B. hyodysenteriae genome was larger than those of the sequenced Borrelia and Treponema species (~09 to 2.8 Mb), but smaller than those of the sequenced Leptospira species (~3.6 to 4.7 Mb). The relatively large size of the B. hyodysenteriae genome likely reflects the spirochete's need for versatility in order to survive in the complex and potentially varying environment of the large intestine. The average G+C content for B. hyodysenteriae WA1 was 27%, which is the lowest average of all sequenced spirochetes, ranging from 28% for Borrelia afzelli  to 52% for Treponema pallidum . The average G+C content of the plasmid was approximately 22%.
Figure 1. Circular representation of the B. hyodysenteriae WA1 genome, with annotated genes.
A, Chromosome (CI); B , Plasmid (PI). Circles range from 1 (outer circle) to 6 (inner circle) for CI and I (outer circle) to IV (inner circle) for PI. Circles 1/I and 2/II, genes and forward and reverse strand; circle 3, tRNA genes; circle 4, rRNA genes, circle 5/III, GC bias/skew ((G-C)/(G+C); red indicates values >0; green indicates values <0); circles 6/IV, A+T percentage content. All genes are color-coded according to Cluster of Orthologous Group (COG) functions: violet for translation, ribosomal structure and biogenesis; plum for RNA processing and modification; pink for transcription; deep pink for DNA replication, recombination and repair; hot pink for chromatin structure and dynamics; wheat for cell division and chromosome partitioning; light salmon for nuclear structure; yellow for defence mechanisms; gold for signal transduction mechanisms; pale green for cell envelope biogenesis, outer membrane; spring green for cell motility and secretion; lawn green for cytoskeleton; yellow green for extracellular structures; aquamarine for intracellular trafficking, secretion, and vesicular transport; medium aquamarine for posttranslational modification, protein turnover, chaperones; cyan for energy production and conversion; deep sky blue for carbohydrate transport and metabolism; sky blue for amino acid transport and metabolism; light slate blue for nucleotide transport and metabolism; orchid for coenzyme metabolism; medium orchid for lipid metabolism; dark orchid for inorganic ion transport and metabolism; blue violet for secondary metabolites biosynthesis, transport and catabolism; slate grey for general function prediction only; grey for function unknown; gray for not in COGS; black for tRNA.doi:10.1371/journal.pone.0004641.g001
The genome contained 2,669 predicted open reading frames (ORFs), and of these there were 2,122 putative protein-coding sequences (CDS) assigned by rpsblast alignment against the NCBI conserved domain database (Table 1), including 273 ORFs with a start codon that was not methionine. This gave the genome a coding density of 86.7%. Of the 2,122 CDS, 1,387 (65%) were assigned a function through sequence analysis. The putative origin of replication consisted of a cluster of CDS comprising dnaA, dnaN, recF and gyrAB. The identity of the origin of replication was supported by GC skew analysis calculated in 10 kb windows across the genome (data not shown). The plasmid contained 31 predicted ORFs of which 26 had an assigned function through sequence similarity and five were hypothetical. The plasmid contained putative genes involved in replication, namely those encoding DnaB-like helicase, DNA primase, and integrase. As it did not contain a dnaA predicted gene, it is likely that this species uses the chromosomally encoded dnaA, and the plasmid-encoded dnaB for replication initiation .
Table 1. General genomic features predicted for B. hyodysenteriae WA1.doi:10.1371/journal.pone.0004641.t001
The three rRNA genes, namely 16S, 23S and 5S rRNA were identified. As previously reported from analysis of a physical map of B. hyodysenteriae strain B78T, the rRNA gene organization in B. hyodysenteriae is unusual . B. hyodysenteriae B78T was shown to have one gene each for 5S (rrf), 16S (rrs), and 23S (rrl) rRNAs. The rrf and rrl genes were closely linked (within 5 kb), with the rrs gene being about 860 kb from the other two rRNA genes. This organization was confirmed in the sequenced strain WA1. A total of 34 transfer RNAs (tRNAs) representing all 20 amino acids were identified. Twelve tRNA genes including tRNA-Met initiator (tRNAi-Met) were found located in the 16S–23S intergenic spacer. The transfer-messenger RNA gene (tmRNA) and four more tRNA genes were upstream of the operon, while downstream were 18 tRNAs including an additional tRNAi-Met and a tRNA-Met elongator (tRNAe-Met). The predicted tRNA genes had an average G+C content of 54% and an average length of 80 bp. A 66 bp domain (62% G+C) of tmRNA (10Sa RNA; transfer messenger RNA) was detected that corresponded to the ssrA gene that encodes tmRNA in all sequenced bacterial genomes. A summary of these general genome statistics is provided in Table 1.
Genes encoding the three subunits of the core RNA polymerase, the σ70 and three alternative σ factors were identified. The nusA, nusB, and rho genes, which are involved in transcription elongation and termination, also were present. The gene for the DNA mismatch repair enzyme was present, but mutH was absent. Except for recBCD, the genome had the complete set of CDS required to perform homologous recombination. Both recA and lexA were present; the latter encodes a repressor that regulates SOS genes .
A total of 140 overlapping gene-pairs were found distributed irregularly within the B. hyodysenteriae genome such that 80% of them occurred on the same strand (unidirectional, →→/←←), and16% occurred on opposite DNA strands (divergent, ←→). The remaining 4% of overlapping genes were convergent. The majority of the unidirectional overlapping regions were relatively short, with >50% of the total observations overlapping by >4 bp. An example was thyA-folA where these two genes overlapped at the 3′ end of thyA (EC.18.104.22.168, COGs0207) and the 5′ end of folA (EC.22.214.171.124, COGs0207), with 4 nucleotides overlapped (ATGA) in frame +3 and +2, respectively. Both genes appeared likely to be transcribed from a single promoter located upstream from thyA. It is likely the thyA-folA overlapping gene-pairs are co-expressed in the folate biosynthesis pathway. In this way, expression of thyA depends on an adjacent gene in a novel way and may reflect the evolution of transcriptional regulation within a gene-dense region. Such overlapping gene-pairs may increase expression levels in response to environment stress.
As previously reported, the B. hyodysenteriae WA1 genome contains a 15 Kb region containing 11 genes encoding structural features of a prophage-like gene transfer agent (GTA) , which is similar to the GTA named VSH-1 that was originally described in B. hyodysenteriae B204 . The GTA is unable to package DNA for its own replication, but it transfers ~7 Kb random genomic fragments between strains of B. hyodysenteriae . Other Brachyspira species contain similar GTAs, although it is not known whether these can transfer genomic fragments between the various Brachyspira species . Two other bacteriophage-like elements were identified in B. hyodysenteriae WA1, and these are briefly discussed later under potential virulence determinants. Four copies of recombinases were found, namely, tyrosine recombinase (xerD: BHWA1_00315), recombinase A (recA: BHWA1_01107), recombinase (BHWA1_01123) and a site-specific recombinase (xerD: BHWA1_02070). An integrase was present on the plasmid, but no other integrases, insertion sequences or transposon-like elements were identified in the genome. This situation is similar to the case in T. pallidum, but is in contrast to the Leptospira genome sequences where large numbers of transposon-like elements are present . From the results it would appear that the novel GTAs are the main mechanism available for gene acquisition in B. hyodysenteriae. Despite this, the genome sequence provided evidence for extensive acquisition of genes from other bacterial genomes (see below).
Comparative genomic analysis
Using the COGs database 48% of the B. hyodysenteriae proteins were classified into three functional groups (Table 2), and only ~10% were assigned to the “poorly characterized” group. Overall, the high proportion of unique and hypothetical proteins meant that COGs functions were assigned to 1,384 (52.2%) out of the 2,669 B. hyodysenteriae CDS features. An overview of the differences between B. hyodysenteriae and other spirochetes is also shown in Table 2. Minor differences were found in all areas, but the two most obvious values where B. hyodysenteriae consistently differed from all the other spirochetes were in signal transduction (T) and in amino acid transport and metabolism (E). The fraction of T proteins was only about 0.8% (21 CDS) of the CDS in B. hyodysenteriae whereas 1.2–1.3% (10–11 CDS) and 2.2–3.3% (80–121 CDS) were found in Borrelia and Leptospira species respectively. The relative paucity of signal transduction mechanisms relative to the genome size may reflect the relatively narrow ecological niche in the porcine large intestine that is occupied by the spirochete. On the other hand, the proportion of genes in the E group in B. hyodysenteriae was 5.6% (148 CDS), which was higher than those in other spirochetes (Table 2). Again this may reflect the environment inhabited by the spirochete, where proteins from host cells and dietary ingredients may be abundant. The different amino acid metabolism may affect nitrogen metabolism in B. hyodysenteriae, which is likely to obtain nitrogen from amino acids.
Table 2. Distribution of Cluster of Orthologous Genes categories in B. hyodysenteriae WA1 and other spirochetes.doi:10.1371/journal.pone.0004641.t002
The similarity of predicted B. hyodysenteriae proteins to those in the 793 complete microbial genomes (as of 27 November 2008) is shown in Table 3. The top sequence matches to the genus of these genomes and the percentage of ORF hits to each are shown. It is noteworthy that 36% and 15% of the B. hyodysenteriae CDS had their best match to proteins from the genus Escherichia (Proteobacteria) and genus Clostridium (Firmicutes) respectively, and only 6% had best matches with other spirochetes. The validity of the best match percentage results was supported by comparing the top five protein matches (Table 3).
Table 3. Similarities of predicted B. hyodysenteriae proteins to those from 716 complete microbial genomes*.doi:10.1371/journal.pone.0004641.t003
Examining the CDS positions along the B. hyodysenteriae genome revealed a number of mini consecutive clusters of 4–5 genes over a 200 Kb region with best matches to various sequenced Clostridium genomes (supplementary Table S1). It is likely that these genes have been involved in horizontal gene transfer events involving B. hyodysenteriae and one or more Clostridium species. Despite being phylogenetically distinct, Brachyspira and Clostridium species are anaerobes with low C+G content, and they inhabit the same environment in the large intestine where there are abundant opportunities for gene exchange that may favor their survival in this niche. Similarly, the facultative anaerobe E. coli co-inhabits this environment, and the extensive protein similarities with B. hyodysenteriae may have resulted from genetic exchange or adaptations to the same environment.
A COGs analysis of the Clostridium-like and Escherichia-like genes (Table 4) indicated that a high proportion of these (52% and 51%, respectively) were involved in metabolism, suggesting their potential role in enhancing growth and survival in the complex nutritional environment of the large intestine. This was consistent with the previously described high ratio of amino acid transport and metabolism COGs pathways in B. hyodysenteriae compared to other genomes (Table 2).
Table 4. COG analysis of Clostridium-like and Escherichia-like genes identified in B. hyodysenteriae WA1.doi:10.1371/journal.pone.0004641.t004
Via COGs analysis, 123 transport CDS were identified in B. hyodysenteriae (supplementary Table S2). These included the three main groups of uptake, efflux, and ATP hydrolysis that energizes the transport. Of these, 72 ABC-type transport CDS were involved in 49 different major primary and secondary transport pathways. The majority of these transport CDS were categorized in the COGs categories E (Amino acid transport and metabolism; 29 CDS), G (Carbohydrate transport and metabolism; 29 CDS), P (Inorganic ion transport and metabolism; 28 CDS) and V (Defence Mechanisms; 10 CDS). Comparative genome analysis showed that B. hyodysenteriae had fewer different “metabolism” proteins spread across clusters G (4.1–4.7%), C (3.1–3.7%) and M (2.8–3.2%) than the Leptospira species (Table 2). However, even though B. hyodysenteriae has a smaller genome than Leptospira, it had a higher percentage of genes involved in core functions, in particular, amino acid transport and metabolism. Furthermore, the same trend was observed in comparison to the smaller genomes of Borrelia and Treponema.
Central metabolic pathway construction
Based on comparisons with enzymes within metabolic pathways from the KEGG  and COGs  databases it was possible to construct the central metabolic pathways in B. hyodysenteriae (Figure 2), with a view to understanding their adaptations for survival in the large intestine. Although pathways for the key cellular processes were reconstructed, a number of pathways were incomplete or absent.
Figure 2. Central metabolic pathway construction for B. hyodysenteriae WA1.doi:10.1371/journal.pone.0004641.g002
The complete repertoire of CDS for glycolysis, gluconeogenesis, a non-oxidative pentose phosphate pathway, nucleotide metabolism, lipopolysaccharide biosynthesis and a respiratory electron transport chain were identified in B. hyodysenteriae WA1. The spirochete did not have a tricarboxylic acid (TCA) cycle, as only two CDS were identified. The absence of a TCA cycle in B. hyodysenteriae, as is in B. burgdorferi, T. pallidum and T. denticola, suggests that ATP is generated by sugar fermentation in these spirochetes , , whereas L. interrogans possesses an electron transport chain and a complete TCA cycle . Substrate level phosphorylation yields stoichiometrically less ATP than oxidative phosphorylation, however the ATP is produced more quickly. The leptospires only possess two endoflagella whilst the other spirochetes have multiple endoflagella, and in these the rapid yield of energy via substrate level phosphorylation may be necessary to fuel their high motility.
It has been reported that B. hyodysenteriae metabolises pyruvate by a clostridial-type clastic reaction to acetyl-CoA, H2, and CO2 . Acetyl CoA is converted to acetate or butyrate as well as ethanol via a branched fermentation pathway. The ATP yielding mechanisms in B. hyodysenteriae WA1 were identified as substrate-level phosphorylation reactions which were mediated by phosphoglycerate kinase (pgk: BHWA1_01680) and pyruvate kinase (pykF: BHWA1_00011) in the glycolysis pathway, and acetate kinase (ackA: BHWA1_00180) that converts acetyl phosphate to acetate. The metabolism of pyruvate reflects the ability of B. hyodysenteriae to survive in the anaerobic environment of the large intestine. The aerobic pyruvate dehydrogenase (aceF: BHWA1_00071) and the anaerobic pyruvate formate lyase (pflX: BHWA1_00810) that are associated with mixed-acid fermentation were present. Conversion of pyruvate to acetyl CoA was aided via three copies of the pyruvate ferrodoxin oxidoreductase gene (por: BHWA1_01937, BHWA1_01938 and BHWA1_01945).
Pathways for the utilization of sugars (glucose, fructose, sucrose, lactose and mannose) and glycerol were identified. B. hyodysenteriae had the capacity to transport several sugars, including sucrose, fructose and glucose, by the phosphotransferase system (PTS). Surprisingly, a complete glycolytic pathway from glucose 6-phosphate to pyruvate was present, and the phosphofructokinase-encoding genes 1-phosphofructokinase (fruK: BHWA1_01536) and 6-phosphofructokinase (pfkA: BHWA1_02459) were found. The enzymes of the glycolysis pathway seemed to be used in gluconeogenesis rather than glycolysis because two copies of fructose bisphosphatase (fbp: BHWA1_01353 and BHWA1_01708), the key enzyme of gluconeogenesis, were present. Therefore, from the reconstruction it appeared that oxidation of amino acids and other non-carbohydrate compounds were used for energy generation by the spirochete via the formation of glucose.
Missing from B. hyodysenteriae were the CDS for two key enzymes for the oxidative pentose phosphate pathway, namely glucose-6-phosphate 1-dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGDH). Other pathways discussed below may be alternatives for energy production.
Catabolic energy producing pathways.
In many bacteria the main pathway for carbohydrate oxidation is the pentose phosphate pathway rather than glycolysis. Ribulose-5-phosphate is an intermediate metabolite in the non-oxidative pentose phosphate pathway that generates NADPH. This serves as the reducing agent in many biosynthetic pathways such as fatty acid and nucleotide biosynthesis. The following putative CDS were identified in B. hyodysenteriae: i) ribulose-5-phosphate epimerase (rep: BHWA1_00002); ii) ribose-5-phosphate isomerase (rpiB: BHWA1_01037); iii) transketolase (tkt: BHWA1_00091 and BHWA1_00092); and iv) transaldolase (BHWA1_01968); these enzymes combine to convert glucose-6-phosphate to ribulose-5-phosphate.
The orthologs for transketolase and ribose-5-phosphate isomerase in the non-oxidative branch were presumed to function to provide the ribose moiety as an important building block for purines and pyrimidines. Although B. hyodysenteriae was predicted to contain the complete complement of non-oxidative pentose phosphate pathway enzymes, based on KEGG analysis it did not utilize this pathway for either ribose-5-phosphate or aromatic amino acid biosynthesis.
Amino acid biosynthesis.
Enzymes involved in the terminal biosynthetic step were found for the nine amino acids serine, glycine, proline, threonine, alanine, lysine, glutamine, aspartate and glutamate. Methionine and cysteine biosynthesis pathways were not identified, and this is consistent with previous findings in other spirochete species , . Aspartate and glutamate amino acid families are involved in biosynthesis and assembly of peptidoglycan, LOS and outer membrane proteins. The deduced metabolism of the amino acid families in B. hyodysenteriae is described in more detail below.
Aspartate derived from oxaloacetate (OAA) is an intermediate precursor in the TCA cycle. B. hyodysenteriae had the capacity to convert L-aspartate to L-lysine through a series of enzymatic reactions including at least four amino acids (glycine, serine, threonine and aspartate) incorporated into pyruvate (Figure 2), which then produced acetate, butyrate and ATP. In addition, B. hyodysenteriae had many amino acid/oligopeptide transporters that would enhance the utilization of amino acids as a major energy source. Diaminopimelate (DAP) is a precursor metabolite for the aspartate biosynthesis pathway and is used for lysine and peptidoglycan biosynthesis in bacteria such as E. coli . Aspartate kinase, the first enzyme in the aspartate metabolism pathway, was encoded by BHWA1_01701 in B. hyodysenteriae. Either LL-2,6-Diaminopimelate (L,LDAP) or meso-DAP isomer or both could be used as an intermediate in this pathway of peptidoglycan synthesis. In addition, meso-DAP is the direct precursor to lysine synthesis in all bacteria . The intermediate precursor of L-lysine serves as a substrate for peptidoglycan synthesis to form an activated precursor molecule . B. hyodysenteriae had a complete pathway for L-lysine synthesis, and also contained genes for cysteine synthase A (cysK: BHWA1_00519) and homoserine O-acetyltransferase (metX: BHWA1_01510) that are involved in synthesizing L-cysteine from L-serine.
D-glutamate is the precursor of other amino acid biosynthesis pathways. One that was present in B. hyodysenteriae WA1 was the glutamate dehydrogenase (gdhA: BHWA1_00140) pathway, in which 2-oxoglutarate undergoes reductive condensation with NH4+, yielding glutamate. 2-oxoglutarate is an intermediate in glutamate biosynthesis from the TCA cycle, which was conserved in B. hyodysenteriae. Previously it has been shown that B. hyodysenteriae possesses GdhA, which is involved in ammonia assimilation by catalyzing the conversion of ammonium and alpha-ketoglutarate to glutamate . Glutamate and glutamine were the primary products of ammonia assimilation, and these amino acids donate nitrogen that is used in biosynthetic reactions . The main metabolic pathway identified for the synthesis of nitrogen into glutamate and glutamine in B. hyodysenteriae involved glutamine synthetase (glnA: BHWA1_00495), glutamate synthetase (gltB: BHWA1_01224) and three copies of oxidoreductase, Fe-S subunit (gltD: BHWA1_00069, BHWA1_00782 and BHWA1_01834). These genes are also found in other Gram-negative bacteria .
Glutamine is formed from glutamate and ammonium by GlnA, which represented a major pathway to assimilate ammonium in B. hyodysenteriae. Glutamate could be formed either by GdhA from 2-oxoglutarate and ammonium, or by GltB. These two enzymes convert glutamine and 2-oxoglutarate into two molecules of glutamate. Due to the presence of glutamate dehydrogenase and the absence of aspartase (AspA), GdhA is probably the major route of ammonia assimilation involving glutamine synthetase and glutamate synthase in B. hyodysenteriae. Glutamate racemase (murL: BHWA1_00378) was present in B. hyodysenteriae and this enzyme produces D-glutamate in an interconversion reaction between D- and L-glutamate, and is responsible for the supply of D-glutamate for the synthesis of peptidoglycan .
In B. hyodysenteriae, threonine, glycine and serine were predicted mainly to be synthesized by standard pathways. Threonine, glycine and serine have a homoserine as an intermediate. The threonine biosynthesis pathway was distinguished by the presence of aspartate kinase (BHWA1_01701) and homoserine dehydrogenase (thrA: BHWA1_00358). However, in E. coli these two enzymes are bifunctional . In B. hyodysenteriae, threonine was synthesized from homoserine by homoserine kinase (ThrB: BHWA1_00610) and threonine synthase (ThrC: BHWA1_00611). Genes for the methionine biosynthesis pathway were not found, as is the case for many other spirochete genomes, although genes encoding a partial methionine salvage pathway were present. The methionine salvage pathway is a ubiquitous biochemical pathway that maintains methionine levels by recycling the thiomethyl moiety of methionine through a degradation pathway that leads from S-adenosylmethionine (SAM) through methylthioadenosine (MTA). It is present in many bacteria, including spirochetes. The pathway in most bacteria involves 11 genes , and four of these genes were identified in B. hyodysenteriae WA1. These were methylthioribose kinase (mtnK; BHWA1_00945), methylthioribose-1-phosphate isomerase (mtnS; BHWA1_00944), 5-methylthioribose-1-phosphate isomerase (mtnA; BHWA1_02491) and S-adenosylhomocysteine nucleosidase (mtnN ; BHWA1_02624). The processes in the methionine salvage pathway are highly dependent on the presence of oxygen and little is known about their functionality when oxygen is scarce or absent. It is probable that an alternative pathway may be utilized in anaerobic bacteria.
Complete pathways for purine and pyrimidine biosynthesis and several components of the salvage pathways for purine and pyrimidine biosynthesis were identified in B. hyodysenteriae.
Genes for de novo synthesis of adenyl and guanisyl phosphates and their deoxy derivatives were present. The capacity for biosynthesis of purines was identified in a series of reactions by addition of functional groups to 5-phosphoribosyl-1-diphosphate, the activated form of ribose-5-phosphate. The absence of enzymes involved in the interconversion of adenine and guanine in B. hyodysenteriae suggested that it depends on purines from host sources or from the immediate environment. Purines may be imported via different subtypes of ATP/ADP translocases including yidC (BHWA1_01539), secA (BHWA1_00872), yajC (BHWA1_01380), secD (BHWA1_01381), secF homolog (BHWA1_01382), putative secE (BHWA1_02319) secY (BHWA1_02143) and secG (BHWA1_02635). Interconversion of pyrimidine nucleotides was feasible in B. hyodysenteriae due to the presence of deoxycytidine triphosphate deaminase (dcd: BHWA1_01136) and FAD-dependent thymidylated synthase (thyA: BHWA1_00171). Cytosine deaminase that converts cytosine into uracil also was present.
Purine nucleoside phosphorylases were expected to complete a metabolic link between nucleoside metabolism and central metabolism to recycle the pentose moiety derived from nucleotides. This linkage may also be functional in purine and pyrimidine salvage, or in the biosynthesis of deoxyribose-1-phosphate. The primary flux of purine nucleotide synthesis occurred via adenosine and hypoxanthine. Adenosine appeared likely to be imported from host cells and converted into inosine by purine nucleoside phosphorylase (deoD: BHWA1_00579). Hypoxanthine also appeared to be derived from the host cell and then converted to inosine-5′-monophosphate (IMP) by hypoxanthine-guanine phosphoribosyltransferase (hpt: BHWA1_01908). IMP serves as the precursor for both AMP and GMP, which are further converted to triphosphates .
Pyrimidine biosynthesis in B. hyodysenteriae appeared to be less complex than in other spirochetes. First, orotate was formed from glutamate and carbamoyl phosphate and was then linked to 5-phosphoribosyl-1-diphosphate and decarboxylated to UMP. Second, UMP was converted to UDP that was converted to CTP, dCTP, dUTP and dTTP. B. hyodysenteriae had deoxyribose-5-phosphate aldolase (deoC: BHWA1_01546), phosphopentomutase (deoB: BHWA1_02377), and two copies of uridine phosphorylase (udp: BHWA1_01547 and BHWA1_01548). These enzymes were expected to complete a metabolic link between nucleoside metabolism and central metabolism to recycle the pentose moiety derived from nucletotides. This linkage also may be functional in purine and pyrimidine salvage, or biosynthesis of deoxyribose-1-phosphate.
B. hyodysenteriae had a capacity for fatty acid and lipid biosynthesis. The spirochete was able to carry out beta-oxidation of long-chain fatty acids. Genes encoding enzymes involved in cleaving and utilizing fatty acid, including long-chain fatty-acid-CoA ligase (fadD: BHWA1_00552) and 3-oxoacyl-ACP (acyl carrier protein) synthase II (fabF: BHWA1_02642) were identified. This is similar to the situation in L. interrogans and T. denticola that both have a complete beta-oxidation pathway , , and suggests that the major energy and carbon source of B. hyodysenteriae is from a common sugar oxidative pathway . Moreover, genes for glycerokinases (glpK: BHWA1_01960), and a glycerol-3-phosphate dehydrogenase (glpF: BHWA1_01961), which is a glycerol uptake facilitator protein, were present in B. hyodysenteriae. These enzymes are involved in glycerol metabolism, suggesting that glycerol and fatty acids may be obtained by this organism through a phospholipid degradation pathway .
Glycerolipid biosynthesis requires the assembly of fatty acids and glycerol. The genes encoding enzymes necessary for the biosynthesis of three glycerolipids were identified in B. hyodysenteriae. Although the first gene (plsB) involved in phospholipids biosynthesis was not identified, B. hyodysenteriae is able to synthesize its own membrane phospholipids . In addition, the spirochete is only able to grow in medium containing lipids, and grows well when cholesterol and phophatidylcholine are included . It is likely that B. hyodysenteriae employs hemolysins to obtain cholesterol, phospholipid glycerol, and other nutrients from host cells .
Cell wall structure biosynthesis
B. hyodysenteriae contains lipooligosaccharide (LOS) as a component of its cell wall . The genome of B. hyodysenteriae was found to contain all three groups of the key genes necessary for LOS biosynthesis, namely a set of genes for lipid A production, core oligosaccharide and O-antigen biosynthesis. At least 77 genes likely to be involved in LOS biosynthesis were found, but unlike other completely sequenced bacterial genomes, such as Leptospira species , these genes were not clustered in a single locus. This may reflect a difference in the B. hyodysenteriae LOS biosynthesis mechanisms.
Lipid A biosynthesis involves the genes lpxA, lpxB, lpxC, lpxD, kdtA, lpxL and lpxM which were all identified in B. hyodysenteriae. Interestingly, lpxH, which is present in E. coli and is important to the structure of lipid A, was not identified. In B hyodysenteriae the absence of lpxH was compensated for by two other CDS: lpxD and lpxB, which likely result in structural differences in lipid A in this organism .
Genes involved in core oligosaccharide biosynthesis also were identified; these included three copies of rfaF (BHWA1_01247, BHWA1_01683 and BHWA1_02595), two copies of rfaE (BHWA1_01926 and BHWA1_00679), and rfaD (BHWA1_00678) for synthesis of the inner core; kdsA (BHWA1_00088), kdsB (BHWA1_01886) and rpe (BHWA1_00002) for the 3-deoxy-D-mann-2-octulsonic acid (Kdo) region of the inner core; and a putative rfaJ (BHWA1_02200), pgi (BHWA1_01137) and galE (BHWA1_01537) for outer core synthesis.
The genes rfbA, rfbB, rfbC and rfbD formed a cluster on the plasmid in the order rfbBADC. A potential promoter was found near the start of the rfbB region, supporting the possibility that the rfb gene cluster was an operon. There was an additional copy of the rfbA gene on the chromosome that was 42 amino acids longer at the 3′ end than the plasmid encoded copy. It is likely that the longer rfbA is the first enzyme involved in LOS biosynthesis as the UDP-N-acetylglucosamine acyltransferase protein domain was contained in the extra 42 amino acids. However, the shorter rfbA on the plasmid was involved in the nucleotide sugar rhamnose biosynthesis pathway. Interestingly, the rfb gene locus spanning 36.7 kb is present on the chromosome of L. borgpetersenii .
The resulting core oligosaccharide attaches to the O-antigen polysaccharide that consists of repeating oligosaccharide units, containing at least one sugar. In B. hyodysenteriae WA1 key enzymes that are necessary for the biosynthesis of an O-specific side chain were present on the plasmid and chromosome. There were two possible intermediates in nucleotide sugar biosynthesis involved in polymerization of sugars in the O-antigen chains. The first, glucose 1-phosphate, was synthesized from the hexose sugars glucose and galactose while the ribose sugar sedpheptulose 7-P was synthesized via the pentose phosphate pathway. Glucose 1- phosphate was used for the biosynthesis of dTDP-rhamnose and involved four genes (rfbA, rfbB, rfbC and rfbD) that were identified on the B. hyodysenteriae plasmid. The rfbC gene product was involved in production of an activated precursor (dTDP-L-rhamnose) necessary for the incorporation of rhamnose moieties into the oligosaccharide backbone of the O-antigen . Sedpheptulose 7-P was used for ADP-L-glycero-D-manno-heptose biosynthesis and involved the genes gmhA, gmhB, rfaE, rfbF and rfbD. The rfbF and rfbD genes were located on the plasmid and the other three putative CDS were only identified on the chromosome. An additional copy of the rfbF gene was found on the chromosome. It was likely that B. hyodysenteriae produced other O-sugars, as additional genes encoding glycosyltransferases were identified; 14 on the chromosome and eight on the plasmid. Further investigations are necessary to determine the nature of the B. hyodysenteriae LOS, especially as the LOS has been implicated as a potential virulence factor.
Four genes (murB, murE, murG and ddlA) involved in peptidoglycan biosynthesis were identified. Interestingly, this organism possessed the genes glmS (BHWA1_01145) and murA (BHWA1_01732) that were involved in the formation of N-acetyl-D-glucosamine that results in N-acetyl-D-glucosamine as a precursor for peptidoglycan biosynthesis. Other key genes, including murC, murD, murF, and mraY that are involved in the final cross-linking of peptidoglycan also were present.
Potential virulence factors
A total of 314 putative virulence factors were screened from all CDS of B. hyodysenteriae using Blast, SignalP, PSORT and TMHMM (Table 5). These were not clustered on identifiable pathogenicity islands. Amongst the putative virulence factors, there were 15 proteases that may be involved in virulence via the destruction of host tissues. This proteolytic capacity was linked with the large number of CDS encoding secreted proteases and enzymes involved in uptake and metabolism of amino acids. An example was a serine protease (BHWA1_00767, BHWA1_01477 and BHWA1_01763) that was found to have >42% sequence identity to a protease of Desulfotalea psychrophila, that in turn was homologous to htrA, an important virulence determinant of Salmonella enterica . The majority of the proteases identified were membrane-bound ATP-dependent Clp proteases (caseinolytic protease) that are well conserved amongst most bacterial species. A number of phospholipases and peptidases also were identified. These enzymes have been suggested to contribute to the pathogenicity of many Gram-negative bacteria.
Table 5. Genes with potential roles in pathogenesis and virulence in B. hyodysenteriae.doi:10.1371/journal.pone.0004641.t005
Other possible virulence factors were 57 CDS for ankyrin proteins, including 39 homologs to those of Trichmonas vaginalis. Ankyrin proteins are known to bind to the host chromatin and could play a critical role in the interaction with the host cell .
The production of hemolysins has been considered a major virulence attribute of B. hyodysenteriae , . B. hyodysenteriae WA1 contained seven potential hemolysin genes, including the four previously reported hemolysin genes encoding hemolysin protein A (tlyA: BHWA1_00238), hemolysin B (tlyB: BHWA1_01228), hemolysin C (tlyC: BHWA1_01427), and an acyl carrier protein (ACP) that contained beta-hemolysin (hylA: BHWA1_02643). The other three genes encoded putative hemolysin III (BHWA1_00446 and BHWA1_01870) and a putative hemolysin CBS domain-containing protein (BHWA1_00587). Interestingly, the hlyA-encoded hemolysin contained a phosphopantetheine binding motif  that binds lipids in a similar way to ACPs. ACPs carry fatty acids as thioester intermediates that are attached to the terminal sulfhydryl group of a phosphopantetheine group, which is then bound to a serine residue . Acylation of toxins is well characterized and essential for activity of RTX toxins . It is likely that B. hyodysenteriae requires an ACP for the expression of a hemolytic phenotype, as has been shown for E. coli . Hemolysin(s) may be involved in the damage to the mucosa that occurs in the large intestine in pigs with swine dysentery.
As mentioned earlier under purine biosynthesis, B. hyodysenteriae WA1 had genes for a Sec pathway. Pathogenic Gram-negative bacteria can have one or more of five other secretion systems, but these were not found in B. hyodysenteriae. Ten flagella- associated genes that can form part of a type III secretory system were found, but needle associated genes (fliC and fliK) that are required for injection of toxins into the host cell were not identified.
Consistent with a high requirement for chemotaxis and motility in the complex environment of the large intestine, the B. hyodysenteriae genome contained at least 84 putative genes associated with these functions, representing approximately 3.5% of the genome. This compared to 6% (63 genes) and 5% (47 genes) for T. pallidum  and Borrelia species  respectively. Overall, 57 of the 84 genes in B. hyodysenteriae were positioned into 17 gene clusters varying in size from 2 to 7 genes. Hence, as in L. interrogans, T. pallidum and B. burgdorferi, the majority of the structural and functional motility and chemotaxis genes were positioned in potential operons , . However, as the B. hyodysenteriae operons were generally small and often only corresponded to parts of the major Leptospira, Treponema and Borrelia operons they probably have undergone extensive rearrangements.
Of the set of 24 core genes involved in flagella biology, 22 were present in B. hyodysenteriae. The exceptions were the flagella assembly genes flgH and flgI which encode the L and P ring proteins in the outer membrane. Pathway analysis revealed that these two genes also are missing in other spirochetes.
Chemotaxis genes can be divided into two groups, methyl-accepting chemotaxis genes (mcp) and chemosensory transducer genes (che). Five classes of mcp genes (aer, tsr, tar, tgr and tap) and nine classes of che genes (cheA, cheB, cheD, cheR, cheV, cheW, cheX, cheY and cheZ) are known to occur. In spirochetes the tsr and tar genes are in high abundance while the aer, trg and tap genes are of low abundance . Seventeen copies of the che genes were found in B. hyodysenteriae: two copies of cheA (BHWA1_00489 and BHWA1_02542), two copies of cheB (BHWA1_00493 and BHWA1_02169), cheC (BHWA1_01397), two copies of cheD (BHWA1_00492 and BHWA1_01642), two copies of cheX (BHWA1_00585 and BHWA1_01332), three copies of cheW (BHWA1_00490, BHWA1_00715 and BHWA1_02278), three copies of cheY (BHWA1_01000, BHWA1_01403 and BHWA1_01333) and two copies of cheR (BHWA1_00491 and BHWA1_01334). It was interesting that there were three copies of the cheY gene as this regulates the rotational direction of the flagella motor . A cheZ homolog was missing, but this also is the case in other spirochetes . All genes (cheR, cheW, cheA, cheY and cheB) were well conserved among B. hyodysenteriae, T. pallidum and B. burgdorferi.
A total of 46 genes encoding methyl-accepting chemotaxis proteins (MCPs) were found in B. hyodysenteriae WA1. The majority of these proteins were encoded by mcpA, mcpB and mcpC. The number of genes encoding methyl-accepting chemotaxis proteins in B. hyodysenteriae was roughly twice as many as in L. interrogans, T. pallidum and B. burgdorferi. The large number of MCPs likely reflects the need for B. hyodysenteriae to transduce numerous chemotactic signals in response to the complex and changing nutritional and physicochemical environment in which it lives. Of the five classes of methyl-accepting chemotaxis genes, B. hyodysenteriae WA1 possessed only three (tsr, tar and trg) whereas T. pallidum possesses four and B. burgdorfori has all five. It is supposed that multiple chemotaxis genes in B. hyodysenteriae and other spirochetes provide redundancy in the system so that they can adapt to diverse environmental conditions. It is likely that signal transduction in these pathogenic spirochetes occurs in a manner very similar to the known pathways. The fact that the B. hyodysenteriae genome contained multiple copies of a number of motility-associated genes partly accounts for the higher number overall compared to other spirochetes.
Compared to other spirochetes, B. hyodysenteriae has undergone a number of adaptations that allow it to survive in the porcine large intestine. It has an anaerobic metabolism and increased numbers of CDSs for carbohydrate and amino acid metabolism and transport, some of which may have been acquired in the intestinal environment. It also has a large number of genes associated with chemotaxis and motility. Genes encoding potential virulence factors such as proteases and hemolysins were identified, but no new pathogenic mechanisms were identified. Analysis of the genome sequences of other pathogenic and non-pathogenic Brachyspira species will aid in confirming the presence of these adaptations, and may help to pinpoint attributes of B. hyodysenteriae that contribute to its ability to cause disease.
Materials and Methods
Spirochete strain and growth conditions
Brachyspira hyodysenteriae strain WA1 (ATCC 49526) was originally isolated in Western Australia from a pig with swine dysentery. The spirochete was colony purified, grown to mid-log phase in pre-reduced anaerobic broth , and a cell pellet prepared.
Genomic DNA preparation and library construction
High molecular weight genomic DNA suitable for preparation of genomic DNA libraries was purified using a cesium chloride gradient following a standard cetyltrimethylammonium bromide (CTAB) extraction method . The genomic DNA was sheared using a GeneMachines Hydroshear, and the fragmented DNA processed for cloning into the pSMART vector system (Lucigen, Middleton, WI, USA). A small insert (2–3 kb) library and a medium insert (3–10 kb) library were constructed into the low copy version of the pSMART vector. Random clones were sequenced using the Applied Biosystems 3730xl DNA Analyzer to provide at least 8× coverage of the genome (see below). The same stock of genomic DNA was used for all the sequencing procedures performed in this study.
Sequencing of the whole genome was undertaken using a Sanger/pyrosequencing hybrid approach through the Australian Genome Research Facility. The first round of sequencing was performed via Sanger sequencing  of the pSMART libraries. A total of 80,051 reads were generated, representing at least 7 times coverage of the whole genome of the spirochete. The second round of high-throughput sequencing was performed using a pyrosequencing approach on a Roche-454 GS20 instrument . A total of two, four-hour runs were performed to generate a total of 384,684 sequences with an average length of about 100 bases, resulting in more than 25 times coverage of the whole genome. The quality filtered reads then were assembled into contiguous sequences using the Newbler Assembler software (http://www.454.com/). Remaining gaps in the genome sequence were closed by PCR walking  between un-linked contiguous sequences to finish the genome sequence.
Sequence analysis and annotation
Open reading frames (ORFs) were predicted using Glimmer 3.02  (National Library of Medicine, Bethesda, MD) using default parameters and a cut-off of 100 nucleotides for coding sequence (CDS) and circular sequence. Artificial CDS within larger CDS were manually removed. Initial annotation involved the detection of tRNA and tmRNA genes using Aragorn 1.1  as well as the identification of the non-coding 5S, 16S and 23S rRNA loci by the BLAST algorithm using BLASTN  and a cut-off value of 1e-05 against known spirochete rRNA sequences in GenBank and other bacterial genomes.
The second stage of annotation used the BLAST program suite  to compare the predicted nucleotide and protein ORF sequences on the CCG (http://ccg.murdoch.edu.au/) Grendel HPC system  against the following databases 1) NCBI non-redundant protein and patent databases (http://www.ncbi.nlm.nih.gov/) 2) String version 7 database  built on Unicellular (COGs) and Eukaryotic Clusters (KOG) of Orthologous Groups; 3) NCBI COGs ; 4) KEGG , and 5) NCBI conserved domain using rpblast . All candidates were selected from the top five alignments with an expected value <1e-05, percent identity >25% and sequence coverage >75%.
Gene assignment was based on the levels of naming specificity used by the Manetee project (The Institute for Genomic Research (TIGR) http://manatee.sourceforge.net). The four levels of gene assignment for CDS were 1) conserved (≥35 per cent identity (PID), ≥75% coverage), 2) known function (30–35 PID, <75% coverage), 3) weak homology (25–30 PID, <75% coverage), and 4) hypothetical (<25 PID, ≥75% coverage).
The predicted protein sequences were further defined with Psort , TMHMM , SignalP  and InterProScan . In-house Perl scripts were used to calculate low and high G+C content. Circular maps of the chromosome and plasmid were produced using Circos 0.48 (http://mkweb.bcgsc.ca/circos/).
The complete nucleotide sequence and annotation of B. hyodysenteriae WA1 has been deposited in GenBank with the accession numbers CPOO1357 for the chromosome and CPOO1360 for the plasmid pBHWA1. Annotations and functional assignments also can be accessed at the CCG website (http://ccg.murdoch.edu.au/).
Mini clusters and duplications of 2–4 genes with best matches to various sequenced Clostridium genomes in B. hyodysenteriae WA1. Shows matches with Clostridial genes
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Transporter genes identified in B. hyodysenteriae WA1. Shows a list of transporter genes
(0.11 MB DOC)
The authors wish to thank Peter J. Wilson and Annette McGrath from the Australian Genome Research Facility for assistance with sequencing and initial bioinformatics analysis.
Conceived and designed the experiments: MIB TL DJH. Performed the experiments: PW KR PM YM DD. Analyzed the data: MIB PW TL KR PM ZA BS YM DD DS AH RB NP DJH. Contributed reagents/materials/analysis tools: AH. Wrote the paper: MIB PW TL DJH.
- 1. Paster BJ, Dewhirst FE (2000) Phylogenetic foundation of spirochetes. J Mol Microbiol Biotechnol 2: 341–344.
- 2. Hampson DJ, Fellström C, Thomson JR (2006) Swine dysentery. In: Straw B, Zimmerman J, Allaire S, Taylor DJ, editors. Diseases of Swine. Oxford: Blackwell. pp. 785–805.
- 3. ter Huurne AA, Gaastra W (1995) Swine dysentery: more unknown than known. Vet Microbiol 46: 347–360.
- 4. Stanton TB, Matson EG, Humphrey SB (2001) Brachyspira (Serpulina) hyodysenteriae gyrB mutants and interstrain transfer of coumermycin A(1) resistance. Appl Environ Microbiol 67: 2037–2043.
- 5. Kennedy MJ, Rosnick DK, Ulrich RG, Yancey RJ Jr (1988) Association of Treponema hyodysenteriae with porcine intestinal mucosa. J Gen Microbiol 134: 1565–1576.
- 6. Milner JA, Sellwood R (1994) Chemotactic response to mucin by Serpulina hyodysenteriae and other porcine spirochetes: potential role in intestinal colonization. Infect Immun 62: 4095–4099.
- 7. Rosey EL, Kennedy MJ, Yancey RJ Jr (1996) Dual flaA1 flaB1 mutant of Serpulina hyodysenteriae expressing periplasmic flagella is severely attenuated in a murine model of swine dysentery. Infect Immun 64: 4154–4162.
- 8. Stanton TB, Rosey EL, Kennedy MJ, Jensen NS, Bosworth BT (1999) Isolation, oxygen sensitivity, and virulence of NADH oxidase mutants of the anaerobic spirochete Brachyspira (Serpulina) hyodysenteriae, etiologic agent of swine dysentery. Appl Environ Microbiol 65: 5028–5034.
- 9. ter Huurne AA, Muir S, van Houten M, van der Zeijst BA, Gaastra W, et al. (1994) Characterization of three putative Serpulina hyodysenteriae hemolysins. Microb Pathog 16: 269–282.
- 10. Halter MR, Joens LA (1988) Lipooligosaccharides from Treponema hyodysenteriae and Treponema innocens. Infect Immun 56: 3152–3156.
- 11. Glockner G, Schulte-Spechtel U, Schilhabel M, Felder M, Suhnel J, et al. (2006) Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity. BMC Genomics 7: 211.
- 12. Fraser CM, Norris SJ, Weinstock GM, White O, Sutton GG, et al. (1998) Complete genome sequence of Treponema pallidum, the syphilis spirochete. Science 281: 375–388.
- 13. Datta HJ, Khatri GS, Bastia D (1999) Mechanism of recruitment of DnaB helicase to the replication origin of the plasmid pSC101. Proc Natl Acad Sci U S A 96: 73–78.
- 14. Zuerner RL, Stanton TB (1994) Physical and genetic map of the Serpulina hyodysenteriae B78T chromosome. J Bacteriol 176: 1087–1092.
- 15. Fraser CM, Casjens S, Huang WM, Sutton GG, Clayton R, et al. (1997) Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi. Nature 390: 580–586.
- 16. Motro Y, La T, Bellgard MI, Dunn DS, Phillips ND, Hampson DJ (2009) Identification of genes associated with prophage-like gene transfer agents in the pathogenic intestinal spirochaetes Brachyspira hyodysenteriae, Brachyspira pilosicoli and Brachyspira intermedia. Vet Microbiol. (in press:doi:10-1016/j.vetmic.2008.09.051).
- 17. Matson EG, Thompson MG, Humphrey SB, Zuerner RL, Stanton TB (2005) Identification of genes of VSH-1, a prophage-like gene transfer agent of Brachyspira hyodysenteriae. J Bacteriol 187: 5885–5892.
- 18. Ren SX, Fu G, Jiang XG, Zeng R, Miao YG, et al. (2003) Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing. Nature 422: 888–893.
- 19. Kanehisa M, Goto S, Kawashima S, Okuno Y, Hattori M (2004) The KEGG resource for deciphering the genome. Nucleic Acids Res 32: D277–280.
- 20. Tatusov RL, Galperin MY, Natale DA, Koonin EV (2000) The COG database: a tool for genome-scale analysis of protein functions and evolution. Nucleic Acids Res 28: 33–36.
- 21. Stanton TB (1989) Glucose metabolism and NADH recycling by Treponema hyodysenteriae, the agent of swine dysentery. Appl Environ Microbiol 55: 2365–2371.
- 22. Seshadri R, Myers GS, Tettelin H, Eisen JA, Heidelberg JF, et al. (2004) Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes. Proc Natl Acad Sci U S A 101: 5646–5651.
- 23. Pavelka MS Jr, Jacobs WR Jr (1996) Biosynthesis of diaminopimelate, the precursor of lysine and a component of peptidoglycan, is an essential function of Mycobacterium smegmatis. J Bacteriol 178: 6496–6507.
- 24. Umbarger HE (1978) Amino acid biosynthesis and its regulation. Annu Rev Biochem 47: 532–606.
- 25. Born TL, Blanchard JS (1999) Structure/function studies on enzymes in the diaminopimelate pathway of bacterial cell wall biosynthesis. Curr Opin Chem Biol 3: 607–613.
- 26. Merrick MJ, Edwards RA (1995) Nitrogen control in bacteria. Microbiol Rev 59: 604–622.
- 27. Magasanik B (1982) Genetic control of nitrogen assimilation in bacteria. Annu Rev Genet 16: 135–168.
- 28. Kada S, Nanamiya H, Kawamura F, Horinouchi S (2004) Glr, a glutamate racemase, supplies D-glutamate to both peptidoglycan synthesis and poly-gamma-glutamate production in gamma-PGA-producing Bacillus subtilis. FEMS Microbiol Lett 236: 13–20.
- 29. Fondi M, Brilli M, Fani R (2007) On the origin and evolution of biosynthetic pathways: integrating microarray data with structure and organization of the common pathway genes. BMC Bioinformatics 8: Suppl 1S12.
- 30. Sekowska A, Dénervaud V, Ashida H, Michoud K, Haas D, Yokota A, Danchin A (2004) Bacterial variations on the methionine salvage pathway. BMC Microbiol 4: 9.
- 31. Rashid N, Imanaka H, Fukui T, Atomi H, Imanaka T (2004) Presence of a novel phosphopentomutase and a 2-deoxyribose 5-phosphate aldolase reveals a metabolic link between pentoses and central carbon metabolism in the hyperthermophilic archaeon Thermococcus kodakaraensis. J Bacteriol 186: 4185–4191.
- 32. Henneberry RC, Cox CD (1970) Beta-oxidation of fatty acids by Leptospira. Can J Microbiol 16: 41–45.
- 33. Nascimento AL, Ko AI, Martins EA, Monteiro-Vitorello CB, Ho PL, et al. (2004) Comparative genomics of two Leptospira interrogans serovars reveals novel insights into physiology and pathogenesis. J Bacteriol 186: 2164–2172.
- 34. Stanton TB, Cornell CP (1987) Erythrocytes as a source of essential lipids for Treponema hyodysenteriae. Infect Immun 55: 304–308.
- 35. Hsu T, Hutto DL, Minion FC, Zuerner RL, Wannemuehler MJ (2001) Cloning of a beta-hemolysin gene of Brachyspira (Serpulina) hyodysenteriae and its expression in Escherichia coli. Infect Immun 69: 706–711.
- 36. Bulach DM, Kalambaheti T, de la Pena-Moctezuma A, Adler B (2000) Lipopolysaccharide biosynthesis in Leptospira. J Mol Microbiol Biotechnol 2: 375–380.
- 37. Babinski KJ, Kanjilal SJ, Raetz CR (2002) Accumulation of the lipid A precursor UDP-2,3-diacylglucosamine in an Escherichia coli mutant lacking the lpxH gene. J Biol Chem 277: 25947–25956.
- 38. Bulach DM, Kalambaheti T, de la Pena-Moctezuma A, Adler B (2000) Functional analysis of genes in the rfb locus of Leptospira borgpetersenii serovar Hardjo subtype Hardjobovis. Infect Immun 68: 3793–3798.
- 39. Xiang SH, Haase AM, Reeves PR (1993) Variation of the rfb gene clusters in Salmonella enterica. J Bacteriol 175: 4877–4884.
- 40. Farn J, Roberts M (2004) Effect of inactivation of the HtrA-like serine protease DegQ on the virulence of Salmonella enterica serovar Typhimurium in mice. Infect Immun 72: 7357–7359.
- 41. Cho NH, Kim JM, Kwon EK, Kim SY, Han SH, et al. (2005) Molecular characterization of a group of proteins containing ankyrin repeats in Orientia tsutsugamushi. Ann N Y Acad Sci 1063: 100–101.
- 42. Magnuson K, Jackowski S, Rock CO, Cronan JE Jr (1993) Regulation of fatty acid biosynthesis in Escherichia coli. Microbiol Rev 57: 522–542.
- 43. Menestrina G, Moser C, Pellet S, Welch R (1994) Pore-formation by Escherichia coli hemolysin (HlyA) and other members of the RTX toxins family. Toxicology 87: 249–267.
- 44. Issartel JP, Koronakis V, Hughes C (1991) Activation of Escherichia coli prohaemolysin to the mature toxin by acyl carrier protein-dependent fatty acylation. Nature 351: 759–761.
- 45. Lux R, Moter A, Shi W (2000) Chemotaxis in pathogenic spirochetes: directed movement toward targeting tissues? J Mol Microbiol Biotechnol 2: 355–364.
- 46. Kuo SC, Koshland DE Jr (1989) Multiple kinetic states for the flagellar motor switch. J Bacteriol 171: 6279–6287.
- 47. Kunkle RA, Harris DL, Kinyon JM (1986) Autoclaved liquid medium for propagation of Treponema hyodysenteriae. J Clin Microbiol 24: 669–671.
- 48. Wilson K (1990) Preparation of genomic DNA from bacteria. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Smith JA, et al., editors. Current Protocols in Molecular Biology. New York: Wiley. pp. 241–242.
- 49. Sanger F, Coulson AR (1975) A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J Mol Biol 94: 441–448.
- 50. Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, et al. (2005) Genome sequencing in microfabricated high-density picolitre reactors. Nature 437: 376–380.
- 51. Delcher AL, Bratke KA, Powers EC, Salzberg SL (2007) Identifying bacterial genes and endosymbiont DNA with Glimmer. Bioinformatics 23: 673–679.
- 52. Laslett D, Canback B (2004) ARAGORN, a program to detect tRNA genes and tmRNA genes in nucleotide sequences. Nucleic Acids Res 32: 11–16.
- 53. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, et al. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389–3402.
- 54. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215: 403–410.
- 55. Hunter A, Schibeci DHLH, Bellgard M (2005) Grendel: A bioinformatics Web Service-based architecture for accessing HPC resources. Newcastle, Australia: Australasian Workshop on Grid Computing and e-Research.
- 56. von Mering C, Jensen LJ, Kuhn M, Chaffron S, Doerks T, et al. (2007) STRING 7–recent developments in the integration and prediction of protein interactions. Nucleic Acids Res 35: D358–362.
- 57. Tatusov RL, Fedorova ND, Jackson JD, Jacobs AR, Kiryutin B, et al. (2003) The COG database: an updated version includes eukaryotes. BMC Bioinformatics 4: 41.
- 58. Kanehisa M, Araki M, Goto S, Hattori M, Hirakawa M, et al. (2008) KEGG for linking genomes to life and the environment. Nucleic Acids Res 36: D480–484.
- 59. Goonesekere NC, Lee B (2008) Context-specific amino acid substitution matrices and their use in the detection of protein homologs. Proteins 71: 910–919.
- 60. Gardy JL, Spencer C, Wang K, Ester M, Tusnady GE, et al. (2003) PSORT-B: Improving protein subcellular localization prediction for Gram-negative bacteria. Nucleic Acids Res 31: 3613–3617.
- 61. Chen Y, Yu P, Luo J, Jiang Y (2003) Secreted protein prediction system combining CJ-SPHMM, TMHMM, and PSORT. Mamm Genome 14: 859–865.
- 62. Bendtsen JD, Nielsen H, von Heijne G, Brunak S (2004) Improved prediction of signal peptides: SignalP 3.0. J Mol Biol 340: 783–795.
- 63. Mulder N, Apweiler R (2007) InterPro and InterProScan: tools for protein sequence classification and comparison. Methods Mol Biol 396: 59–70.